Images from the past reveals that Dr. James Marcus, founder of Umbrella Corporation originally had a girl who was dying of premature aging diseases. Desperate to save her, Marcus developed the T-virus to cure all illnesses existing on the planet. But seeing his stolen creation, business partner Marcus Alexander Dr. Isaacs (Iain Glen) tried to persuade him to provide virus-T army to use it for military purposes.
When he refused to do so, Isaacs and ordered Albert Wesker (Shawn Roberts) to kill Marcus. Flash forward to now, three weeks after the events of the previous film, Alice (Milla Jovovich) wakes up in the White House who is now ruined, after being betrayed by Wesker still once. While searching for survivors, the Red Queen (Ever Gabo Anderson) makes an appearance and says he must return to nest in Raccoon City in the next 48 hours as Umbrella Corporation has developed there a virus that has the ability to kill any T-carrier of the virus.
Alice asks why the Red Queen betrays its creator and it answers that will say after you get there. On the way to Raccoon City, Alice is captured by Isaacs, but manages to escape his convoy. Later, when she arrives in Raccoon, Alice finds the destroyed city and is captured by a group of surviving humans consists of Doc (Eoin Macken), Abigail (Ruby Rose), Christian (William Levy), Cobalt (Rola), Razor (Fraser James ) and Claire Redfield (Ali Larter) – the latter being presumed dead after attack in Arcadia, but managed to escape alive.